Short-term storage of cane toad (Bufo marinus) gametes
This study is summarised as evidence for the following.
Freeze sperm or eggs for future useAction Link
Freeze sperm or eggs for future use
A replicated study of captive cane toads Bufo marinus in Australia (Browne, Clulow & Mahony 2001) found that storage method and temperature affected sperm and egg viability. Sperm stored in testes showed greater than 50% motility for seven days at 0°C and five days at 4°C. By day 15 only sperm stored at 0°C showed any motility (3%). In suspension, the longest retention of motility and fertilizing capacity was following storage in concentrated (1:1 dilution) anaerobic suspensions (up to 25–30 days). However, fertilization rates were significantly higher following storage in 1:5 dilution (day 5: 85% vs 55% for other concentrations). Egg viability was significantly higher following storage at 15°C compared to other temperatures (8 hours: 90% vs 0–60%). Storage at 5°C resulted in a decline to 0% viability after two hours. Sperm from wild toads were stored in intact testes at 0 or 4°C for 15 days (n = 6/treatment) or in suspension (macerated testes; n = 24) with Simplified amphibian Ringer solution at 0°C for 30 days. Dilutions were 1:1, 1:5 or 1:10 (testes:solution) and storage tubes were either opened or sealed. Immediately after ovulation, eggs from three females were stored in simplified amphibian Ringer solution at 5, 10, 15, 20 and 25°C (1,500 eggs/female). Fertilization rate was monitored up until 12 hours.