Study

Actions

This study is summarised as evidence for the following.

Action Category

Freeze sperm or eggs for future use

Action Link
Amphibian Conservation
  1. Freeze sperm or eggs for future use

    A replicated study in 1997–1998 of captive amphibians in the USA (Beesley, Costanzo & Lee 1998) found that recovery of viable sperm following freezing was significantly lower for leopard frogs Rana pipiens and American toads Bufo americanus compared to freeze-tolerant wood frogs Rana sylvatica. Sperm recovery was 59%, 48% and 81% respectively. Survival and viability of wood frog sperm was significantly greater using the cryoprotectant dimethyl sulfoxide and supplement of fetal bovine serum (survival: 96%; viability: 45%) than the other three protectants with glutathione (survival: 34–54%; viability with methanol: 10%) or without protectants (survival: 44–54%; viability with methanol: 16%). Testes from wild or commercially obtained males were macerated in a buffer solution. Sperm solutions from wood frogs were mixed with 0.5 M cryoprotectant (dimethyl sulfoxide, methanol, glycerol or ethylene glycol), a supplement (fetal bovine serum or glutathione) or a combination of these. Using the most successful cryopreservation treatment, sperm from each species was incubated on ice for 15 minutes, then frozen to -80°C for 1 hour (rate: 130°C/minute). Thawing was in warm water.

     

     

Output references

What Works in Conservation

What Works in Conservation provides expert assessments of the effectiveness of actions, based on summarised evidence in synopses. Subjects covered so far include amphibians, birds, terrestrial mammals, forests, peatland and control of freshwater invasive species. More are in progress.

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